NOS3

Cilia effects upon perturbation of NOS3

Ciliogenesis screen results (4 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-13.00) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

abnormal spleen morphology, enlarged lymph nodes, abnormal epididymis morphology, decreased circulating phosphate level, abnormal lymph node morphology, enlarged spleen, hyperplasia, decreased lymphocyte cell number, increased blood urea nitrogen level, increased eosinophil cell number, increased basophil cell number, lymphoid hyperplasia, decreased fasting circulating glucose level, enlarged epididymis, increased neutrophil cell number

Mouse ciliopathy phenotype:

abnormal kidney morphology, small kidney, increased heart weight

Subcellular localization

basal body, cytosol, microtubules, nucleus

Functional category

• Ciliary assembly/disassembly
• Actin & cytoskeleton regulation
• Reproduction & sperm
• Cell migration & adhesion
• Signaling (Hedgehog, GPCRs, ion channels)
• Cardiac & muscle development
• Muscle contraction & physiology

Function

endothelial NOS (eNOS, NOS3)) eNOS reportedly localises to the basal body of cilia to modulate CBF sig lling via cGMP-PKG and cAMP-PKA(20110742, 25460324).