NOS3

nitric oxide synthase 3

Tier: Gold Standard Ciliary Genes

Ensembl:
ENSG00000164867
UniProt:
P29474
OMIM:
163729
Synonyms:
ECNOS, ENOS

Ciliogenesis screen results (4 screens)

  • Kim2016: Not Reported
  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-13.00) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:
abnormal spleen morphology, enlarged lymph nodes, abnormal epididymis morphology, decreased circulating phosphate level, abnormal lymph node morphology, enlarged spleen, hyperplasia, decreased lymphocyte cell number, increased blood urea nitrogen level, increased eosinophil cell number, increased basophil cell number, lymphoid hyperplasia, decreased fasting circulating glucose level, enlarged epididymis, increased neutrophil cell number

Subcellular localization

basal body, cytosol, microtubules, nucleus

References: PMID:20110742, PMID:25460324

Functional category

  • Ciliary assembly/disassembly
  • Actin & cytoskeleton regulation
  • Reproduction & sperm
  • Cell migration & adhesion
  • Signaling (Hedgehog, GPCRs, ion channels)
  • Cardiac & muscle development
  • Muscle contraction & physiology

Function

endothelial NOS (eNOS, NOS3)) eNOS reportedly localises to the basal body of cilia to modulate CBF signalling via cGMP-PKG and cAMP-PKA(20110742, 25460324).