NRP1

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, casTLE effect=-1.05) PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

preweaning lethality, complete penetrance, decreased prepulse inhibition

Subcellular localization

cilia, cytosol

References: PMID:28667171, doi:10.1101/2025.05.11.653368

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Actin & cytoskeleton regulation
• Neurogenesis & migration
• Viral interactions
• Cell migration & adhesion
• Signaling (Hedgehog, GPCRs, ion channels)
• Cardiac & muscle development
• Axon guidance & growth
• Transcription regulation

Function

The Neuropilins (NRPs), a small family comprised of NRP1 and NRP2, have well-established roles in axon guidance and vascular patterning (25244320) and act to positively regulate HH signaling at the cell surface (22051878). NRPs are expressed in a variety of HH-responsive tissues during critical periods of HH-related developmental patterning (16846494). NRPs are novel class of cell surface HH pathway regulators that act downstream of ligand binding through cytoplasmic effectors to control HH pathway function(28667171). Detected in primary cilia of human hypothalamic neurons by proximity proteomics (Macarelli et al., 2025, bioRxiv, doi 10.1101/2025.05.11.653368).