NRP1

Cilia effects upon perturbation of NRP1

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, casTLE effect=-1.05) PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

preweaning lethality, complete penetrance, decreased prepulse inhibition

Mouse ciliopathy phenotype:

abnormal auditory brainstem response

Subcellular localization

cilia, cytosol

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Actin & cytoskeleton regulation
• T cell biology
• Neurogenesis & migration
• Viral interactions
• Cell migration & adhesion
• Signaling (Hedgehog, GPCRs, ion channels)
• Cardiac & muscle development
• Axon guidance & growth
• Transcription regulation

Function

The Neuropilins (NRPs), a small family comprised of NRP1 and NRP2, have well-established roles in axon guidance and vascular patterning (25244320) and act to positively regulate HH sig ling at the cell surface (22051878). NRPs are expressed in a variety of HH-responsive tissues during critical periods of HH-related developmental patterning (16846494). NRPs are novel class of cell surface HH pathway regulators that act downstream of ligand binding through cytoplasmic effectors to control HH pathway function(28667171).