NUBP1

NUBP iron-sulfur cluster assembly factor 1, cytosolic

Ensembl:
ENSG00000103274
UniProt:
P53384
OMIM:
600280
Synonyms:
CIAO5, NBP1, NBP35

Cilia effects upon perturbation of NUBP1

Cilia number / % ciliated:
Incrased cilia number

Ciliogenesis screen results (4 screens)

  • Kim2016: Not Reported
  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-10.69) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Subcellular localization

basal body, cytosol

Functional category

  • Ciliary assembly/disassembly
  • Actin & cytoskeleton regulation
  • Metabolism
  • T cell biology

Function

Nubp1 and Nubp2 interact with each other, but their individual knockdown, however, has differential effects on centrosome and spindle formation: while Nubp1 silencing qualitatively phenocopies KIFC5A silencing, also resulting in significant centrosome amplification throughout the cell cycle and the formation of multipolar spindles, Nubp2 appears to have a modulatory/accessory role for centriole arithmetics, in concert with KIFC5A and Nubp1 [16638812]. Nubp1 and Nubp2 localize on interphase centrioles in cycling cells and basal bodies in quiescent cells(23807208).

Model organism evidence

Mus musculus (2 references)

We further demonstrate that in vivo these isoforms are able to interact with themselves, with each other and moreover directly and independently with MRP/MinD-type P-loop NTPases Nubp1 and Nubp2, which are integral components of centrioles, negative regulators of ciliogenesis and implicated in ce

The nucleotide-binding proteins Nubp1 and Nubp2 are negative regulators of ciliogenesis.

PMIDs: 26153462, 23807208

C. elegans (1 reference)

The nucleotide-binding proteins Nubp1 and Nubp2 are negative regulators of ciliogenesis.

PMIDs: 23807208

Xenopus (1 reference)

Xenopus laevis nucleotide binding protein 1 (xNubp1) is important for convergent extension movements and controls ciliogenesis via regulation of the actin cytoskeleton.

PMIDs: 23685253