NUBP2

NUBP iron-sulfur cluster assembly factor 2, cytosolic

Ensembl:
ENSG00000095906
UniProt:
Q9Y5Y2
OMIM:
610779
Synonyms:
CFD1, CIAO6

Cilia effects upon perturbation of NUBP2

Cilia number / % ciliated:
Incrased cilia number

Ciliogenesis screen results (3 screens)

  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-5.94) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: Increased Signaling (Negative Regulator) PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:
increased circulating fructosamine level, preweaning lethality, complete penetrance, decreased total reti thickness
Mouse ciliopathy phenotype:
short tibia

Subcellular localization

basal body, centrosome, nucleus

Functional category

  • Ciliary assembly/disassembly
  • T cell biology

Function

Nubp1 and Nubp2 interact with each other, but their individual knockdown, however, has differential effects on centrosome and spindle formation: while Nubp1 silencing qualitatively phenocopies KIFC5A silencing, also resulting in significant centrosome amplification throughout the cell cycle and the formation of multipolar spindles, Nubp2 appears to have a modulatory/accessory role for centriole arithmetics, in concert with KIFC5A and Nubp1 [16638812]. Nubp1 and Nubp2 localize on interphase centrioles in cycling cells and basal bodies in quiescent cells(23807208).

Model organism evidence

Mus musculus (4 references)

Premature differentiation of neurons is associated with defects in the centrosome and/or primary cilia.

NUBP2 is implicated in both the cytosolic iron/sulfur cluster assembly pathway and negative regulation of ciliogenesis.

PMIDs: 39867373, 31733190, 26153462, 23807208

C. elegans (1 reference)

The nucleotide-binding proteins Nubp1 and Nubp2 are negative regulators of ciliogenesis.

PMIDs: 23807208