NUBP2

Cilia effects upon perturbation of NUBP2

Cilia number / % ciliated:

Incrased cilia number

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-5.94) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: Increased Signaling (Negative Regulator) PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

increased circulating fructosamine level, preweaning lethality, complete penetrance, decreased total reti thickness

Subcellular localization

basal body, centrosome, nucleus

References: PMID:16638812, PMID:23807208

Functional category

• Ciliary assembly/disassembly

Function

Nubp1 and Nubp2 interact with each other, but their individual knockdown, however, has differential effects on centrosome and spindle formation: while Nubp1 silencing qualitatively phenocopies KIFC5A silencing, also resulting in significant centrosome amplification throughout the cell cycle and the formation of multipolar spindles, Nubp2 appears to have a modulatory/accessory role for centriole arithmetics, in concert with KIFC5A and Nubp1 [16638812]. Nubp1 and Nubp2 localize on interphase centrioles in cycling cells and basal bodies in quiescent cells(23807208).