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Phenotypes

Mouse phenotype:

short tibia, small adrenal glands, abnormal head shape, abnormal facial morphology, abnormal cranium morphology, increased circulating bilirubin level, abnormal retina blood vessel morphology

Subcellular localization

Ciliary associated gene, Cytosol

Functional category

• Metabolism
• Ciliary assembly/disassembly

Function

We examined four genes up-regulated and not previously known to be involved with cilia (ZMYND10, NXN, GLOD4, SPATA4) by knockdown of the human orthologs in human retinal pigment epithelial cells (hTERT-RPE1) cells to ask whether they are involved in cilia-related processes that include cilia assembly, cilia length control, basal body/centriole numbers, and the distance between basal bodies/centrioles. All of the genes have cilia-related phenotypes and, surprisingly, our data show that knockdown of GLOD4 and SPATA4 also affects the cell cycle. These results demonstrate that whole-genome transcriptome analysis during ciliogenesis is a powerful tool to gain insight into the molecular mechanism by which centrosomes and cilia are assembled(23604077).