NXN

Phenotypes

Mouse phenotype:

short tibia, small adrenal glands, abnormal head shape, abnormal facial morphology, abnormal cranium morphology, increased circulating bilirubin level, abnormal retina blood vessel morphology

Subcellular localization

Cytosol

References: PMID:23604077

Functional category

• Metabolism
• Ciliary assembly/disassembly

Function

We examined four genes up-regulated and not previously known to be involved with cilia (ZMYND10, NXN, GLOD4, SPATA4) by knockdown of the human orthologs in human retinal pigment epithelial cells (hTERT-RPE1) cells to ask whether they are involved in cilia-related processes that include cilia assembly, cilia length control, basal body/centriole numbers, and the distance between basal bodies/centrioles. All of the genes have cilia-related phenotypes and, surprisingly, our data show that knockdown of GLOD4 and SPATA4 also affects the cell cycle. These results demonstrate that whole-genome transcriptome analysis during ciliogenesis is a powerful tool to gain insight into the molecular mechanism by which centrosomes and cilia are assembled(23604077).