ODF4

outer dense fiber of sperm tails 4

Ensembl:
ENSG00000184650
UniProt:
Q2M2E3
OMIM:
610097
Synonyms:
CT136, OPPO1

Cilia effects upon perturbation of ODF4

Loss-of-function effect:
Shorter cilia

Ciliogenesis screen results (5 screens)

  • Kim2016: Not Reported
  • Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381
  • Pusapati et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, neg_rank=712, lfc=-1.42) PMID:30270045

Phenotypes

Mouse ciliopathy phenotype:
abnormal uriry bladder morphology, enlarged heart, male infertility

Subcellular localization

flagella

Functional category

  • Reproduction & sperm

Function

ODF4 has localized in flagella.

Model organism evidence

Mus musculus (2 references)

RESULTS: In in vitro condition, Odf4 -/- spermatozoa with hairpin flagella harboring large cytoplasmic droplets (CDs) underwent capacitation, about 30% of large CDs were removed from spermatozoa, and the flagella became straight (capacitation test).

The association of ODF4 with AK1 and AK2 in mice is essential for fertility through its contribution to flagellar shape.

PMIDs: 39314832, 36804949