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Subcellular localization

basal body, Nucleoplasm, Plasma membrane

Functional category

• Metabolism
• Ciliary assembly/disassembly

Function

This study demonstrates that OGT (O-linked N-acetylglucosamine transferase) is required for both primary and motile ciliogenesis in mice. Using OGT haploinsufficient mice, the authors showed that reduced OGT expression and decreased protein O-GlcNAcylation lead to significant ciliary defects, including fewer and shorter photoreceptor primary cilia and tracheal motile cilia. Tracheal tissues also exhibited a substantial reduction in the number of ciliated cells. Localization studies in multiple cell types revealed that OGT accumulates around the basal body/centrosome, the site from which cilia emerge. High-resolution 3D-SIM microscopy further demonstrated that OGT localizes to the outer layer of the pericentriolar material (PCM), adjacent to centrosomal proteins such as CDK5RAP2, pericentrin, and γ-tubulin(32607788).