PDCD5

Cilia effects upon perturbation of PDCD5

Cilia number / % ciliated:

Decreased cilia number

Loss-of-function effect:

Shorter cilia

Ciliogenesis screen results (4 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.18) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

increased mean corpuscular volume

Mouse ciliopathy phenotype:

abnormal kidney morphology

Subcellular localization

cilia associated gene, cytosol, nucleoplasm

Functional category

• Ciliary assembly/disassembly; Motile cilium & axoneme

Function

PDCD5 is required for flagellum biogenesis and ciliogenesis and present the PDCD5-TRiC structures in their open states at near-atomic resolution. Mechanically, we find that PDCD5 promotes substrates release by competing with PhLP2A to interact with TRiC, and the depletion of PDCD5 traps flagellum- and cilium-associated proteins within TRiC, finally leading to malformed flagella of spermatids and cilia in mouse ciliated cells. PDCD5 is required for both flagellum biogenesis and ciliogenesis. PDCD5 promotes substrate release from open-state TRiC.Pdcd5 knockout leads to protein retention in the cytoplasm of elongating spermatids(PMID: 41506263).