PIK3R4

phosphoinositide-3-kinase regulatory subunit 4

Ensembl:
ENSG00000196455
UniProt:
Q99570
OMIM:
602610
Synonyms:
P150, VPS15

Cilia effects upon perturbation of PIK3R4

Cilia number / % ciliated:
No effect
Loss-of-function effect:
Shorter cilia

Ciliogenesis screen results (4 screens)

  • Kim2016: No effect
  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.31) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: Hyper-ciliogenesis (robust z=2.43, ciliated=56.2%) PMID:26595381

Ciliopathy associations

  • Nephronophthisis
  • Retinal Dystrophy/Degeneration

Subcellular localization

basal body, cytosol

Functional category

  • Ciliary assembly/disassembly
  • Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
  • Viral interactions
  • Protein processing & maturation
  • Signaling (Hedgehog, GPCRs, ion channels)

Function

Also known as VPS15. Interacts with the golgin GM130 to form a complex and ensure efficient loading of IFT20 in vesicles addressed from the cis-Golgi and transported to the basal body of the cilium (27882921).

Model organism evidence

Danio rerio (1 reference)

A mutation in VPS15 (PIK3R4) causes a ciliopathy and affects IFT20 release from the cis-Golgi.

PMIDs: 27882921