PKN2

protein kinase N2

Tier: Gold Standard Ciliary Genes

Ensembl:
ENSG00000065243
UniProt:
Q16513
OMIM:
602549
Synonyms:
PAK-2, PRK2, PRKCL2, STK7

Ciliogenesis screen results (2 screens)

  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.94) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, casTLE effect=-2.01) PMID:29459680

Phenotypes

Mouse phenotype:
decreased circulating hdl cholesterol level, preweaning lethality, complete penetrance, decreased circulating unsaturated transferrin level, decreased circulating total protein level, decreased exploration in new environment, decreased circulating cholesterol level, increased startle reflex

Subcellular localization

centrosome, cilia, nucleus, transition zone

References: PMID:32127582

Functional category

  • Ciliary assembly/disassembly
  • Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
  • Viral interactions
  • Cell migration & adhesion
  • Transition zone

Function

pPRK1/pPRK2 localise to the transition zone of cilia and their co-depletion results in reduced cilia size, impaired planer polarity and impaired cilia associated signalling(32127582). PKN2 is phosphorylated by CDK10/Cyclin M; PKN2 loss destabilizes RhoA, and this pathway restrains ciliogenesis through actin network formation (PMID 27104747).