PKN2

Cilia effects upon perturbation of PKN2

Ciliogenesis screen results (2 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.94) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, casTLE effect=-2.01) PMID:29459680

Phenotypes

Mouse phenotype:

decreased circulating hdl cholesterol level, preweaning lethality, complete penetrance, decreased circulating unsaturated transferrin level, decreased circulating total protein level, decreased exploration in new environment, decreased circulating cholesterol level, increased startle reflex

Mouse ciliopathy phenotype:

increased circulating bilirubin level

Subcellular localization

centrosome, cilia, nucleus, transition zone

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Viral interactions
• Cell migration & adhesion
• Transition zone

Function

pPRK1/pPRK2 localise to the transition zone of cilia and their co-depletion results in reduced cilia size, impaired planer polarity and impaired cilia associated sig lling(32127582).