PLCZ1

Cilia effects upon perturbation of PLCZ1

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-5.61) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

increased fasting circulating glucose level; decreased circulating iron level; decreased mean platelet volume; abnormal gait

Human ciliopathy phenotype:

Male infertility with spermatogenesis disorder due to single gene mutation

Ciliopathy associations

• Male Infertility

Subcellular localization

flagella

Functional category

• Metabolism; Reproduction & sperm

Function

The alteration of the gene or protein expression of PLCZ1 results in low or no fertility. In human assisted reproduction, PLCZ1 characterization is of essential importance when oocyte activation failures are linked to male infertility. However, little is known about the importance of PLCZ1 for in vivo and in vitro fertility outcomes in cattle and horses, particularly as the commercial use of assisted fertilization grows. In horses, intracytoplasmic sperm injection (ICSI) is the elective method for assisted fertilization. Sperm PLCZ1 amount exhibits a large variation from stallion to stallion which can influence fertility outcomes. Stallion sperm samples showing low PLCZ1 consistently result in lower fertility after ICSI. In contrast, ICSI is not efficient in cattle due to the inconsistent ability of bull sperm to activate bovine oocytes. Interestingly, the experimental microinjection of exogenous PLCZ1 is able to rescue oocytes that failed to activate, even reaching live birth.In noncapacitated stallion sperm, PLCZ1 is localized in the acrosomal and equatorial regions, and particularly in the connecting piece and principal piece of the tail(PMID: 38133249).