PRKACA

protein kinase cAMP-activated catalytic subunit alpha

Tier: Gold Standard Ciliary Genes

Ensembl:
ENSG00000288516
UniProt:
P17612
OMIM:
601639
Synonyms:
PKACA

Cilia effects upon perturbation of PRKACA

Overexpression effect:
Increased

Ciliogenesis screen results (5 screens)

  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.41) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381
  • Pusapati et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, neg_rank=517, lfc=-1.07) PMID:30270045
  • Elliott et al. 2025 (CRISPRa) [CRISPRa]: Disassembly Trigger (casTLE Effect=-4.35) PMID:41160700

Ciliopathy associations

  • Cardioacrofacial Dysplasia 1
  • Ellis-van Creveld Syndrome
  • Skeletal Ciliopathy

References: PMID:35927022, PMID:33875766, PMID:38357848, PMID:33058759

Subcellular localization

basal body, centrosome, cilia, cytosol, mitochondria, nucleus, ciliary tip

References: doi:10.1371/journal.pbio.3003902, PMID:41005307, PMID:30598432, PMID:39743588

Functional category

  • Ciliary assembly/disassembly
  • Actin & cytoskeleton regulation
  • Metabolism
  • Reproduction & sperm
  • Viral interactions
  • Protein processing & maturation
  • Signaling (Hedgehog, GPCRs, ion channels)
  • Cardiac & muscle development
  • Muscle contraction & physiology
  • Transcription regulation

Function

Catalytic subunit of PKA. At centriolar satellites. Part of a multimeric complex nucleated by PCM1. Phosphorylates NEK10 for CHIP-mediated ubiquitination and proteolysis resulting in cilia resorption. Patched1-ArhGAP36-PKA- Inversin axis determines the ciliary translocation of Smoothened for Sonic Hedgehog pathway activation. 30598432 Doganli et al. 2026 additionally show prominent PKA-Calpha localization at the ciliary base and at the ciliary tip in RPE-1 cells, where it acts with TAB2 as an upstream activator of TAK1, phosphorylating TAK1 at S412 at the ciliary base in response to TGFB1 and BMP2 during cardiomyogenesis. DOI 10.1371/journal.pbio.3003902 Identified by cryo-EM as a component of radial spoke 3 in sperm flagella and other motile cilia (Leung et al., 2025, Nature, PMID 39743588).