PRKAR1B

protein kinase cAMP-dependent type I regulatory subunit beta

Tier: Gold Standard Ciliary Genes

Ensembl:
ENSG00000188191
UniProt:
P31321
OMIM:
176911

Ciliogenesis screen results (4 screens)

  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.84) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381
  • Pusapati et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, neg_rank=953, lfc=-1.24) PMID:30270045

Phenotypes

Human ciliopathy phenotype:
primary ciliary dyskinesia

Subcellular localization

cilia, cytosol

References: PMID:18687752, PMID:39743588

Functional category

  • Ciliary assembly/disassembly
  • Metabolism
  • Viral interactions
  • Signaling (Hedgehog, GPCRs, ion channels)

Function

P2Y12 purinergic receptor, adenylyl cyclases (i.e., AC4, AC6, and AC8), and protein kinase A (i.e., PKA RI-β and PKA RII-α regulatory subunits), exchange protein directly activated by cAMP (EPAC) isoform 2, and A-kinase anchoring proteins (i.e., AKAP150) are expressed in cholangiocyte cilia. Identified by cryo-EM as a component of radial spoke 3 in sperm flagella and other motile cilia (Leung et al., 2025, Nature, PMID 39743588).