PTGER4

prostaglandin E receptor 4

Ensembl:
ENSG00000171522
UniProt:
P35408
OMIM:
601586
Synonyms:
EP4

Cilia effects upon perturbation of PTGER4

Ciliogenesis screen results (4 screens)

  • Kim2016: No effect
  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-5.71) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: Hyper-ciliogenesis (robust z=2.18, ciliated=54.3%) PMID:26595381

Subcellular localization

basal body, cilia

Functional category

  • Ciliary assembly/disassembly
  • Actin & cytoskeleton regulation
  • T cell biology
  • Cell migration & adhesion
  • Signaling (Hedgehog, GPCRs, ion channels)
  • Cilia length regulation

Function

Also known as EP4. Activates cAMP-mediated sig ling cascade. Together with COX- 2, is required for cilia formation and elongation. Increases the intracellular cAMP and activates protein ki se A (PKA) sig ling (25173977). We observed that depletion of ABCC4 or EP4 in hRPE1 cells caused a reduction of ciliaformation and elongation when compared with use of the control siRNA (Fig. 7a, b, c). Bothcilia length and percentages of ciliated cells were significantly reduced in ABCC4 or EP4 depleted cells (Fig. 7g. h). Addition of exogenous PGE2 increased both cilia length and percentages of ciliated cells in control cells but not in EP4-depleted cells (Fig. 7a, d, g, h), indicating that EP4 acts downstream of PGE2 signaling during ciliogenesis.

Model organism evidence

Mus musculus (1 reference)

Transport of GPCRs to primary cilia requires TULP3, whose knockdown in primary human and mouse islets relocalized ciliary FFAR4 and PTGER4 and impaired regulated glucagon or insulin secretion, without affecting ciliary structure.

PMIDs: 34385262