RAB10

RAB10, member RAS oncogene family

Ensembl:
ENSG00000084733
UniProt:
P61026
OMIM:
612672

Cilia effects upon perturbation of RAB10

Cilia number / % ciliated:
Decreased cilia number
Loss-of-function effect:
Shorter cilia
Overexpression effect:
No effect

Ciliogenesis screen results (3 screens)

  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-3.48) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:
iris synechia, decreased mean corpuscular volume, preweaning lethality, complete penetrance, decreased mean corpuscular hemoglobin, increased startle reflex, embryonic lethality prior to organogenesis
Mouse ciliopathy phenotype:
abnormal auditory brainstem response

Subcellular localization

basal body

Functional category

  • Ciliary assembly/disassembly
  • Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
  • Actin & cytoskeleton regulation

Function

Negative regulator of ciliogenesis. Rab10 phosphorylation enhances binding to RILPL1, which in turn strengthens its ability to block ciliogenesis (30398148). Mediates membrane protein trafficking (21856246). Interacts in a protein complex with Sec8 at the basal body (20576682).

Model organism evidence

Mus musculus (3 references)

Activating leucine-rich repeat kinase 2 (LRRK2) mutations cause Parkinson's and phosphorylation of Rab10 by pathogenic LRRK2 blocks primary ciliogenesis in cultured cells.

We previously reported that Tbc1d21-null mice exhibit severe sperm tail defects with a disrupted axoneme, and that TBC1D21 interacts with RAB10.

PMIDs: 39088390, 38822685, 37874635