RAB10

Cilia effects upon perturbation of RAB10

Cilia number / % ciliated:

Decreased cilia number

Loss-of-function effect:

Shorter cilia

Overexpression effect:

No effect

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-3.48) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

iris synechia, decreased mean corpuscular volume, preweaning lethality, complete penetrance, decreased mean corpuscular hemoglobin, increased startle reflex, embryonic lethality prior to organogenesis

Subcellular localization

basal body

References: PMID:20576682

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Actin & cytoskeleton regulation

Function

Negative regulator of ciliogenesis. Rab10 phosphorylation enhances binding to RILPL1, which in turn strengthens its ability to block ciliogenesis (30398148). Mediates membrane protein trafficking (21856246). Interacts in a protein complex with Sec8 at the basal body (20576682).