RAB5A

Cilia effects upon perturbation of RAB5A

Cilia number / % ciliated:

No effect

Loss-of-function effect:

No effect

Overexpression effect:

No effect

Ciliogenesis screen results (4 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

increased erythrocyte cell number, increased circulating sodium level, abnormal sleep behavior, decreased grip strength, increased circulating potassium level, decreased mean corpuscular volume, decreased mean corpuscular hemoglobin, decreased circulating hdl cholesterol level

Mouse ciliopathy phenotype:

abnormal reti morphology, increased heart weight

Subcellular localization

basal body

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Viral interactions
• Signaling (Hedgehog, GPCRs, ion channels)
• Ciliary membrane

Function

Localisation of the clathrin light chain, AP-2 clathrin adaptor, dynamin and RAB-5 endocytic proteins overlaps with a morphologically discrete periciliary membrane compartment associated with sensory cilia(PMID: 22342749). STAM-1 and RAB-5 collocate in the cell bodies and ciliary bases of polycystin-expressing neurons (Ppkd-2::STAM-1A:: GFP and Ppkd-2::mRFP::RAB-5, Figure 3, B–D). Combined, these data indicate that C. elegans STAM-1A colocalizes with HGRS-1 on the early endosome at the ciliary base, where they may participate in endocytic sorting of LOV-1 and PKD-2(17581863).

Model organism evidence