RAB8A

Cilia effects upon perturbation of RAB8A

Loss-of-function effect:

Shorter cilia

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-3.24) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

increased grip strength, decreased lean body mass

Subcellular localization

basal body, centrosome, cilia

References: PMID:41005307, PMID:17574030, PMID:22433857, PMID:24213529, PMID:32669361

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Actin & cytoskeleton regulation
• Signaling (Hedgehog, GPCRs, ion channels)
• Ciliary membrane

Function

Rab8 targets vesicles to the cilium and promotes ciliary membrane elongation. It is activated by Rabin8 (17574030, 22433857). Inactivating Rab8 (GDP- bound state) blocks ciliogenesis and induces BBS phenotypes in zebrafish (17574030). Rab8a, Rab8b and Rab10 work synergistically for ciliogenesis (24213529). Rab8a specifically interacts with cenexin/ODF2, a basal body and microtubule binding protein required for cilium biogenesis (17646400). Regulates the trafficking of rhodopsin in photoreceptors. Interacts with RPGR which regulates its activity and localisation. Involved in polarised trafficking of vesicles from the trans-Golgi network (20631154). Involved in serum-starvation-induced ciliogenesis (32669361)