?

Subcellular localization

Ciliary associated gene

Functional category

• Non-motile cilium / primary cilium

Function

With respect to Ral isoform involvement, we find RalA localizing to the basal body of primary cilia, colocalizing with γ-tubulin upon starvation of MEFs, whereas RalB was found at vesicular structures in the cytoplasm (Figs 6H and S6L), which might indicate that primary cilium formation is specifically controlled by the RalA isoform. RalA localization to the primary cilium was not altered upon loss of RalGAPβ or κB-Ras1/2 (Fig S6M), suggesting that the RalGAP:κB-Ras complex likely controls activity and not subcellular localization of RalA.. We also observed hampered primary cilium formation in κB-Ras DKO MEFs upon starvation (Fig 6E and F), demonstrating that this function of RalGAP/Ral signaling requires the presence of κB-Ras proteins. Knockdown of RalA and RalB in WT and RGβKO MEFs reduced primary cilium formation (Fig S6H and I)(40490362).