RAN

RAN, member RAS oncogene family

Ensembl:
ENSG00000132341
UniProt:
P62826
OMIM:
601179
Synonyms:
ARA24, GSP1, TC4

Cilia effects upon perturbation of RAN

Loss-of-function effect:
Longer cilia

Ciliogenesis screen results (4 screens)

  • Kim2016: Not Reported
  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-6.85) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Subcellular localization

basal body, cilia, cytosol, microtubules, nucleus

Functional category

  • Ciliary assembly/disassembly
  • Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
  • Actin & cytoskeleton regulation
  • T cell biology
  • Reproduction & sperm
  • Viral interactions
  • Transcription regulation

Function

Ran GTP regulates ciliary protein transport and ciliogenesis (21998203). RanGTP gradient regulates trafficking and entry of kinesin motor KIF17 into cilium. It has been proposed that Ran plays a role in regulating cellular compartmentalisation by controlling the shuttling of cytoplasmic proteins into ciliary compartments (20526328). Interacts with Importin 尾2, which interacts directly with cargoes, releasing them upon binding Ran GTP and regulating localisation of RP2 (21285245).

Model organism evidence

Mus musculus (2 references)

Mechanistic investigation reveals that intraflagellar transport 88 (IFT88), a protein essential for ciliary assembly and maintenance, undergoes UFMylation at lysine 572.

Light and electron microscopy showed numerous defects in the head-to-tail coupling apparatus of the sperm, and a small number of structural defects in the sperm flagella.

PMIDs: 41272290, 40373254