RAN

Cilia effects upon perturbation of RAN

Loss-of-function effect:

Longer cilia

Ciliogenesis screen results (4 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-6.85) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Subcellular localization

basal body, cilia, cytosol, microtubules, nucleus

References: PMID:21998203, PMID:20526328, PMID:21285245

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Actin & cytoskeleton regulation
• Reproduction & sperm
• Viral interactions
• Transcription regulation

Function

Ran GTP regulates ciliary protein transport and ciliogenesis (21998203). RanGTP gradient regulates trafficking and entry of kinesin motor KIF17 into cilium. It has been proposed that Ran plays a role in regulating cellular compartmentalisation by controlling the shuttling of cytoplasmic proteins into ciliary compartments (20526328). Interacts with Importin β2, which interacts directly with cargoes, releasing them upon binding Ran GTP and regulating localisation of RP2 (21285245).