RHO

Cilia effects upon perturbation of RHO

Ciliogenesis screen results (4 screens)

• Kim2016: No effect
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-5.29) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: Joubert Candidate / Ciliogenesis Defect (robust z=-2.66, ciliated=17.3%) PMID:26595381

Phenotypes

Human ciliopathy phenotype:

retinitis pigmentosa

Ciliopathy associations

• Retinal Dystrophy/Degeneration

Subcellular localization

basal body, cilia

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Reproduction & sperm
• Protein processing & maturation
• Signaling (Hedgehog, GPCRs, ion channels)
• Ciliary membrane

Function

Cilia-specific GPCR essential for vision. Multiple roles in trafficking, interacts with Arf4, TcTex-1 and SARA (23966685). Mutations cause protein misfolding and retention in the endoplasmic reticulum, leading to cellular stress and cell death. Mutations are associated with retinitis pigmentosa (32343782).

Model organism evidence