RHOA

ras homolog family member A

Ensembl:
ENSG00000067560
UniProt:
P61586
OMIM:
165390
Synonyms:
ARH12, ARHA, RHO12, RHOH12

Cilia effects upon perturbation of RHOA

Loss-of-function effect:
Longer cilia

Ciliogenesis screen results (5 screens)

  • Kim2016: Not Reported
  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-3.28) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: Increased Signaling (Negative Regulator) PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: Hyper-ciliogenesis (robust z=2.05, ciliated=53.3%) PMID:26595381
  • Elliott et al. 2025 (CRISPRa) [CRISPRa]: Disassembly Trigger (casTLE Effect=-2.09) PMID:41160700

Subcellular localization

basal body, cytosol, nucleus

Functional category

  • Ciliary assembly/disassembly
  • Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
  • Actin & cytoskeleton regulation
  • T cell biology
  • Protein processing & maturation
  • Cell migration & adhesion
  • Signaling (Hedgehog, GPCRs, ion channels)
  • Cardiac & muscle development
  • Muscle contraction & physiology

Function

p190A specifically at the basal body to promote ciliogenesis through a local dampening of Rho GTPase-dependent F-actin polymerization. In both wild type and Arhgap35D34/D34 proximal tubules Rac1 and RhoA are enriched at the basal bodies to similar degrees (26859289).

Model organism evidence

Mus musculus (2 references)

Neddylation relieves cytoskeletal tension to permit primary cilia formation during mouse decidualization.

Loss of primary cilia triggers RhoA-MLC2-dependent actomyosin contraction, which transmits mechanical forces to the nuclear lamina, leading to micronuclei formation.

PMIDs: 41709324, 40229503

Xenopus (1 reference)

This disruption was rescued by transient expression of constitutively active RhoA in multiciliated cells.

PMIDs: 40141151