RHOA

ras homolog family member A

Tier: Gold Standard Ciliary Genes

Ensembl:
ENSG00000067560
UniProt:
P61586
OMIM:
165390
Synonyms:
ARH12, ARHA, RHO12, RHOH12

Cilia effects upon perturbation of RHOA

Loss-of-function effect:
Longer cilia

Ciliogenesis screen results (5 screens)

  • Kim2016: Not Reported
  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-3.28) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: Increased Signaling (Negative Regulator) PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: Hyper-ciliogenesis (robust z=2.05, ciliated=53.3%) PMID:26595381
  • Elliott et al. 2025 (CRISPRa) [CRISPRa]: Disassembly Trigger (casTLE Effect=-2.09) PMID:41160700

Subcellular localization

basal body, cytosol, nucleus

References: PMID:26859289

Functional category

  • Ciliary assembly/disassembly
  • Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
  • Actin & cytoskeleton regulation
  • Protein processing & maturation
  • Cell migration & adhesion
  • Signaling (Hedgehog, GPCRs, ion channels)
  • Cardiac & muscle development
  • Muscle contraction & physiology

Function

p190A specifically at the basal body to promote ciliogenesis through a local dampening of Rho GTPase-dependent F-actin polymerization. In both wild type and Arhgap35D34/D34 proximal tubules Rac1 and RhoA are enriched at the basal bodies to similar degrees (26859289). RhoA acts downstream of CDK10/Cyclin M and PKN2 to build the actin network that restrains ciliogenesis (PMID 27104747).