RILPL1

Rab interacting lysosomal protein like 1

Ensembl:
ENSG00000188026
UniProt:
Q5EBL4
OMIM:
614092
Synonyms:
FLJ39378

Cilia effects upon perturbation of RILPL1

Cilia number / % ciliated:
Incrased cilia number
Loss-of-function effect:
Longer cilia

Ciliogenesis screen results (2 screens)

  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.24) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680

Phenotypes

Mouse phenotype:
increased startle reflex, increased fasting circulating glucose level

Subcellular localization

basal body, centrosome, cilia, cytosol, nucleus

Functional category

  • Ciliary assembly/disassembly
  • Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
  • Actin & cytoskeleton regulation
  • Ciliary membrane

Function

Rab effector-related protein, regulates protein concentration by removal of membrane proteins from the cilium. Depletion results in accumulation of sig ling proteins in the ciliary membrane, including Smo. Interacts with activated Rab34, Rab36, Rab12 and Rab40B (23264467). Interacts with the LRRK2- phosphorylated forms of Rab8A and Rab10 (29125462). Enhances pRab10 clustering on pericentriolar membranes to suppress ciliogenesis (30398148).

Model organism evidence

Mus musculus (2 references)

Rab10 phosphorylation strengthens its intrinsic ability to block ciliogenesis by enhancing binding to RILPL1.

Affinity enrichment mass spectrometry revealed that the primary ciliogenesis regulator, RILPL1 specifically interacts with the LRRK2-phosphorylated forms of Rab8A and Rab10, whereas RILPL2 binds to phosphorylated Rab8A, Rab10, and Rab12.

PMIDs: 30398148, 29125462