SIRT2

Cilia effects upon perturbation of SIRT2

Cilia number / % ciliated:

Decreased cilia number

Loss-of-function effect:

Longer cilia

Overexpression effect:

Decreased

Ciliogenesis screen results (5 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381
• Elliott et al. 2025 (CRISPRa) [CRISPRa]: Disassembly Trigger (casTLE Effect=-3.66) PMID:41160700

Subcellular localization

centrosome, cilia, cytosol, microtubules, nucleus

References: PMID:32213867, PMID:24203696

Functional category

• Ciliary assembly/disassembly
• Actin & cytoskeleton regulation
• Reproduction & sperm
• Neurogenesis & migration
• Protein processing & maturation
• Signaling (Hedgehog, GPCRs, ion channels)
• Cardiac & muscle development
• Transcription regulation

Function

Regulates cell cycle progression at mitosis, replication and G1/S transition. SIRT2 acts as a sensor that links growth factor-dependent signal transduction and primary cilia formation by regulating the cell cycle under nutrient- present conditions. Suppression leads to a cell cycle arrest and promotes formation of primary cilia, by inhibiting mTOR signaling mainly via mTORC1 (32213867). Functions as a tubulin deacetylase. Regulates ciliogenesis and centrosome amplification. Inhibition of SIRT2 induces hyperacetylation of α-tubulin and blocks cilia dissaembly. SIRT2 could also be responsible for aberrant centrosome amplification and polyploidy in polycystic kidney disease (24203696).