SP5

Cilia effects upon perturbation of SP5

Cilia number / % ciliated:

Decreased cilia number

Loss-of-function effect:

Shorter cilia

Ciliogenesis screen results (4 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.08) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

decreased hematocrit, decreased fasting circulating glucose level, hyperactivity, increased grip strength, increased vertical activity, abnormal vibrissa morphology

Subcellular localization

nucleus

References: PMID:40875857

Functional category

• Ciliary assembly/disassembly
• Transcription regulation

Function

TFs SP5 and SP8 regulate cilia formation and gene expression. In embryos lacking Sp5 and Sp8, primary and motile cilia were shorter than normal and reduced in number across cell types, contributing to situs inversus and hydrocephalus(PMID: 40875857). SP5 knockout mice show shorter and fewer primary and motile cilia; contributes to situs inversus and hydrocephalus. SP5 regulates cilia formation and gene expression as a transcription factor.