SPAG16

Ciliogenesis screen results (2 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680

Ciliopathy associations

• Primary Ciliary Dyskinesia

References: PMID:34445527

Subcellular localization

cilia, nucleus

References: PMID:16382026, PMID:17699735, PMID:21655194, PMID:28137312

Functional category

• Ciliary assembly/disassembly
• Actin & cytoskeleton regulation
• Reproduction & sperm
• Cell migration & adhesion
• Transcription regulation

Function

Orthologue of Chlamydomonas reinhardtii PF20, known to be essential to the structure and function of the motile axoneme. Encodes two proteins, SPAG16L and SPAG16S. SPAG16L is essential for sperm flagellar motility. Interacts with SPAG6, which in turn interacts with SPAG17. Mutation results in instability of interacting proteins in the central pair of the sperm axoneme. (16382026, 17699735). SPAG16S, identified only in male germ cells, plays a role in the gene expression machinery of male germ cells required for spermatogenesis, and it also modulates Spag16L mRNA expression (21655194). Transcription of SPAG16 is regulated through S-SOX5 binding to the promoter (28137312).