SPAG16

sperm associated antigen 16

Ensembl:
ENSG00000144451
UniProt:
Q8N0X2
OMIM:
612173
Synonyms:
DKFZP666P1710, FLJ22724, PF20, WDR29

Cilia effects upon perturbation of SPAG16

Ciliogenesis screen results (2 screens)

  • Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680

Ciliopathy associations

  • Primary Ciliary Dyskinesia

Subcellular localization

cilia, nucleus

Functional category

  • Ciliary assembly/disassembly
  • Actin & cytoskeleton regulation
  • T cell biology
  • Reproduction & sperm
  • Cell migration & adhesion
  • Transcription regulation

Function

Orthologue of Chlamydomo s reinhardtii PF20, known to be essential to the structure and function of the motile axoneme. Encodes two proteins, SPAG16L and SPAG16S. SPAG16L is essential for sperm flagellar motility. Interacts with SPAG6, which in turn interacts with SPAG17. Mutation results in instability of interacting proteins in the central pair of the sperm axoneme. (16382026, 17699735). SPAG16S, identified only in male germ cells, plays a role in the gene expression machinery of male germ cells required for spermatogenesis, and it also modulates Spag16L mR expression (21655194). Transcription of SPAG16 is regulated through S-SOX5 binding to the promoter (28137312).

Model organism evidence

Mus musculus (3 references)

In the testis, SPAG6 was detected in spermatogonia, spermatocytes, and in the sperm flagella whereas SPAG16 was found in spermatocytes and in the sperm flagella.

BACKGROUND: The mammalian sperm-associated antigen 16 gene (Spag16) uses alternative promoters to produce two major transcript isoforms (Spag16L and Spag16S) and encode proteins that are involved in the cilia/flagella formation and motility.

PMIDs: 30911868, 28137312, 25762510