SPEN

Cilia effects upon perturbation of SPEN

Loss-of-function effect:

Decreased cilia number

Ciliogenesis screen results (1 screen)

• Breslow et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, casTLE effect=-0.65) PMID:29459680

Subcellular localization

cilia associated

Functional category

• T cell biology
• Non-motile cilium / primary cilium
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)

Function

SPEN reexpression in T47D cells containing a nonsense mutation in SPEN restored the primary cilium, whereas its knockdown in MCF10A and Hs578T cells considerably decreased primary cilia levels. We also report that SPEN regulates migration in breast cells, but only in those harboring primary cilia, and that KIF3A silencing, a critical factor in primary cilia, partially reverses SPEN's effects, suggesting that SPEN may coordinate cellular movement through primary cilia-dependent mechanisms(28877752).