SREBF1

Cilia effects upon perturbation of SREBF1

Cilia number / % ciliated:

Decreased cilia number

Loss-of-function effect:

Shorter cilia

Overexpression effect:

Not Reported, Reduced cilia numbers

Ciliogenesis screen results (5 screens)

• Kim2016: No effect
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-5.90) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: Hyper-ciliogenesis (robust z=2.68, ciliated=58.2%) PMID:26595381
• Kim et al. 2010 (siRNA) [siRNA]: Longer Cilia (Area per Cilia z=5.64) PMID:20393562

Subcellular localization

cytosol, nucleus

References: PMID:20889723, PMID:25904332

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Signaling (Hedgehog, GPCRs, ion channels)
• Muscle contraction & physiology
• Transcription regulation

Function

SREBP1c-induced loss of the primary cilium could be confirmed in mammalian Madin-Darby canine kidney (MDCK) cells and was mediated by changes in the supply of fatty acids(20889723, 25904332)., Also known as SREBP1. Suppresses primary ciliogenesis. Induces large levels of lysophosphatidylcholine and lysophosphatidylethanolamine , which are typically generated at the outer membrane leaflet. It increases the positive membrane curvature in the plasma membrane and vesicle membranes, causing Rab11- positive vesicles to deviate away from the pericentrosomal preciliary compartment at the ciliary base, suppresing ciliogenesis ( 25904332).