TGIF2

Cilia effects upon perturbation of TGIF2

Cilia number / % ciliated:

Decreased cilia number

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-5.39) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

preweaning lethality, complete penetrance

Mouse ciliopathy phenotype:

decreased circulating calcium level

Subcellular localization

basal body, nucleus

Functional category

• Ciliary assembly/disassembly
• Signaling (Hedgehog, GPCRs, ion channels)
• Transcription regulation

Function

Reducing Evi5l expression in MEFs lacking Tgifs resulted in a partial restoration of cilium numbers and in the transcriptio l response to activation of the Shh pathway.TGIF2 localized to basal body (25613900)

Model organism evidence