TMEM97

Cilia effects upon perturbation of TMEM97

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Pusapati et al. 2018 (CRISPR) [CRISPR]: Negative Regulator (Hh signaling, pos_rank=77, lfc=0.86) PMID:30270045

Phenotypes

Mouse phenotype:

decreased hemoglobin content, abnormal vocalization, decreased hematocrit, decreased erythrocyte cell number, increased red blood cell distribution width

Mouse ciliopathy phenotype:

small testis

Subcellular localization

cilia

Functional category

• Ciliary assembly/disassembly
• Signaling (Hedgehog, GPCRs, ion channels)

Function

TMEM97 is a sterol-binding tetraspanin membrane receptor required for cellular cholesterol homeostasis. We previously reported immunolocalization to photoreceptor (PR) connecting cilia in mouse retina (Fliesler et. al. ARVO 2022), but questioned the unexpected pattern observed. Here, we further evaluated TMEM97 immunolocalization in the mouse retina, comparing multiple TMEM97 antibodies and improving antigen presentation/target specificity.These findings are consistent with TMEM97 having a role in the structure and function of primary cilia in neural retina (especially photoreceptors) and RPE centrosomes.