TNRC6A

Cilia effects upon perturbation of TNRC6A

Cilia number / % ciliated:

Decreased cilia number

Loss-of-function effect:

Shorter cilia

Ciliogenesis screen results (2 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.75) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680

Subcellular localization

basal body, cytosol, p-body

References: PMID:18653466, PMID:21880135

Functional category

• Ciliary assembly/disassembly
• Signaling (Hedgehog, GPCRs, ion channels)
• Transcription regulation

Function

Two stationary GW/P bodies reside at the centrosome in human malignant cells [18653466] raises the possibility that miRNA could potentially be located at the centrosome in GW/P bodies and may play a role in posttranscriptio l regulation of mRNA involved in centrosome functions including primary ciliogenesis. Two GW/P bodies as marked by GW182 and hAgo2 colocalized to the basal body of primary human astrocytes as well as human synoviocytes during interphase and specifically with the distal end of the basal body in the pericentriolar region. knock down of GW182, hAgo2, Drosha and DGCR8 mRNA did not affect the appearance of the earliest stage of ciliogenesis but did prevent the formation and elongation of the ciliary axoneme(21880135).