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Phenotypes

Mouse phenotype:

abnormal retina morphology, male infertility, abnormal auditory brainstem response, female infertility

Subcellular localization

basal body

Functional category

• Metabolism

Function

TRIM37 localizes to basal bodies and cell junctions in multiciliated cells and is associated with centrosomal and ciliary regulatory functions(29180514). The minor elevation in cells with increased centriole number was suppressed by expression of WT, but not Ligmut, TRIM37 (Fig. 1 D). Expansion microscopy makes it straightforward to assess centriole overduplication by counting the number of procentrioles per mother centriole in S phase cells. Based on two independent experiments scoring >1,000 S phase cells with duplicating mother centrioles, no centriole overduplication was observed in TRIM37Δ or in TRIM37Δ cells expressing Ligmut TRIM37. Thus, the ∼5% of cells with extra centrioles observed in TRIM37Δ cells do not arise from overduplication in S phase. TRIM37Δ RPE1 cells also retained the ability to assemble primary cilia following serum starvation (Fig. 1 E). Ciliation frequency and ciliary length were slightly elevated in the TRIM37Δ cell line relative to parental RPE1 cells (Fig. 1 E and Fig. S1 A), however, whether this results from TRIM37 loss or reflects clonal variation is unclear(33983387).