TRIP6

Cilia effects upon perturbation of TRIP6

Cilia number / % ciliated:

Decreased cilia number

Loss-of-function effect:

Shorter cilia

Ciliogenesis screen results (4 screens)

• Kim2016: No effect
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-6.21) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: Hyper-ciliogenesis (robust z=3.08, ciliated=61.2%) PMID:26595381

Phenotypes

Mouse phenotype:

increased respiratory quotient, enlarged spleen

Mouse ciliopathy phenotype:

hydrocephaly, abnormal cranium morphology

Subcellular localization

cilia, nucleus

Functional category

• Ciliary assembly/disassembly
• Actin & cytoskeleton regulation
• Signaling (Hedgehog, GPCRs, ion channels)
• Transcription regulation

Function

The requirement for homodimerization which doubles its interaction sites, its punctate localization along the axoneme, and its co-localization with other cilia components suggest a scaffold/co-transporter function for TRIP6 in cilia(34620853).