TTLL6

Cilia effects upon perturbation of TTLL6

Cilia number / % ciliated:

No effect

Loss-of-function effect:

Decrease

Ciliogenesis screen results (2 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.59) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680

Phenotypes

Mouse phenotype:

increased circulating potassium level, decreased circulating glucose level

Subcellular localization

basal body, cilia, flagella

References: PMID:41410385, PMID:41005307, PMID:21262966, PMID:22246503, PMID:23897886

Functional category

• Ciliary assembly/disassembly
• Actin & cytoskeleton regulation
• Cell migration & adhesion

Function

Main axonemal tubulin glutamylase of ciliated cells. Knockdown caused loss of axonemal glutamylation but not basal body glutamylation (21262966). Ciliary entry and distribution of TTLL6 is regulated by CEP41 (22246503). Critical in the regulation of axonemal motility and ciliary beating in ependymal cells, as it cannot be replaced by any of the other glutamylases (23897886). TTLL6 glutamylase preferentially modifies α-tubulin tails in microtubules via quadrivalent recognition spanning two tubulin dimers. Implicated in ciliopathies via defective axoneme polyglutamylation.