UBE2E1

Cilia effects upon perturbation of UBE2E1

Cilia number / % ciliated:

Decreased cilia number

Loss-of-function effect:

Shorter cilia

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-4.13) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

increased monocyte cell number, increased mean corpuscular volume, increased mean platelet volume, increased hematocrit, decreased mean corpuscular hemoglobin concentration

Mouse ciliopathy phenotype:

abnormal reti morphology

Subcellular localization

basal body, cytosol, nucleus

Functional category

• Ciliary assembly/disassembly
• Viral interactions

Function

Correct 尾-catenin levels are tightly regulated at the primary cilium by a ciliary-specific E2 (UBE2E1) and a regulatory substrate-adaptor (MKS1)(35170427).