VAMP7

Cilia effects upon perturbation of VAMP7

Loss-of-function effect:

decreased ciliation

Overexpression effect:

Shorter cilia

Ciliogenesis screen results (1 screen)

• Wheway et al. 2015 (siRNA) [siRNA]: Hyper-ciliogenesis (z=2.72) PMID:26167766

Subcellular localization

cilia associated

Functional category

• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Ciliary assembly/disassembly

Function

VAMP7 knockdown had no effect on apical delivery of numerous cargoes tested, but did decrease the length and frequency of primary cilia. Additionally, VAMP7 knockdown disrupted cystogenesis in cells grown in a three-dimensional basement membrane matrix. The effects of VAMP7 depletion on ciliogenesis and cystogenesis are not directly linked to the disruption of lysosomal function, as cilia lengths and cyst morphology were unaffected in an MDCK lysosomal storage disorder model. Together, our data suggest that VAMP7 plays an essential role in ciliogenesis and lumen formation. To our knowledge, this is the first study implicating an R-SNARE in ciliogenesis and cystogenesis(24466086).