WDR44

Cilia effects upon perturbation of WDR44

Cilia number / % ciliated:

Decreased cilia number

Loss-of-function effect:

Shorter cilia

Overexpression effect:

Decreased, Not Reported

Ciliogenesis screen results (4 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Ciliopathy associations

• WDR44-related X-linked Ciliopathy

References: PMID:39235309, PMID:38191484

Subcellular localization

cytosol, endosome

References: PMID:31204173, PMID:31665227, PMID:38191484

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)

Function

Rab11 binding protein, negatively regulates ciliogenesis. Akt phosphorylates WDR44 at Ser342/344 to stabilise it and increase its binding to Rab11, which prevents ciliogenesis. WDR44 depletion promotes binding of Rab11a to Rabin8, which leads to preciliary trafficking and ciliogenesis- initiating events at the mother centriole. Disruption of Akt signaling causes a switch from Rab11-WDR44 to the Rab11-FIP3-Rabin8 complex which promotes ciliogenesis(31204173) Endosomal protein phosphorylated by Akt at Ser346 (31665227)