WDR44

WD repeat domain 44

Ensembl:
ENSG00000131725
UniProt:
Q5JSH3
OMIM:
301070
Synonyms:
DKFZP686L20145, RAB11BP, RPH11, SYM-4

Cilia effects upon perturbation of WDR44

Cilia number / % ciliated:
Decreased cilia number
Loss-of-function effect:
Shorter cilia
Overexpression effect:
Decreased, Not Reported

Ciliogenesis screen results (4 screens)

  • Kim2016: Not Reported
  • Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Ciliopathy associations

  • Joubert Syndrome

Subcellular localization

cilia, cytosol

Functional category

  • Ciliary assembly/disassembly
  • Trafficking (BBSome, small GTPases, vesicular transport, ATPases)

Function

Rab11 binding protein, negatively regulates ciliogenesis. Akt phosphorylates WDR44 at Ser342/344 to stabilise it and increase its binding to Rab11, which prevents ciliogenesis. WDR44 depletion promotes binding of Rab11a to Rabin8, which leads to preciliary trafficking and ciliogenesis- initiating events at the mother centriole. Disruption of Akt sig ling causes a switch from Rab11-WDR44 to the Rab11-FIP3-Rabin8 complex which promotes ciliogenesis(31204173) Endosomal protein phosphorylated by Akt at Ser346 (31665227)

Model organism evidence

Mus musculus (1 reference)

We establish BLTP2 as a negative regulator of ciliogenesis in RPE-1 cells based on a strong genetic interaction with WDR44, a gene that also suppresses ciliogenesis.

PMIDs: 38536441

Drosophila (1 reference)

We establish BLTP2 as a negative regulator of ciliogenesis in RPE-1 cells based on a strong genetic interaction with WDR44, a gene that also suppresses ciliogenesis.

PMIDs: 38536441