WNT3A

Cilia effects upon perturbation of WNT3A

Cilia number / % ciliated:

Decreased cilia number

Overexpression effect:

No effect

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

rib fusion, increased eosinophil cell number, abnormal spleen morphology, vertebral transformation, abnormal rib morphology, abnormal vertebral arch morphology, increased circulating triglyceride level, abnormal coat appearance, abnormal coat/ hair morphology, preweaning lethality, complete penetrance, abnormal vertebrae morphology, vertebral fusion

Subcellular localization

cilia

References: PMID:16291790, PMID:32023461, PMID:36878953

Functional category

• Ciliary assembly/disassembly
• Actin & cytoskeleton regulation
• Neurogenesis & migration
• Cell migration & adhesion
• Signaling (Hedgehog, GPCRs, ion channels)
• Cardiac & muscle development
• Axon guidance & growth
• Transcription regulation

Function

Wnt3a may also modulate the symmetry-breaking activity of mechanosensory cilia in the node. Wnt3a links the segmentation clock and AP axis elongation with key left-determining events, suggesting that Wnt3a is an integral component of the trunk organizer ( 16291790). Wnt3a treatment promotes primary ciliogenesis in hTERT-RPE cells ( 32023461). WNT3A may also act by increasing the number, strength or coherence of beating cilia (PMID 36878953).