WNT4

Cilia effects upon perturbation of WNT4

Ciliogenesis screen results (4 screens)

• Kim2016: No effect
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.06) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: Hyper-ciliogenesis (robust z=2.84, ciliated=59.4%) PMID:26595381

Phenotypes

Mouse phenotype:

decreased circulating HDL cholesterol level; thrombocytopenia; increased neutrophil cell number; increased circulating bilirubin level; increased monocyte cell number; decreased circulating cholesterol level; decreased lymphocyte cell number; increased circulating aspartate transaminase level

Subcellular localization

cilia associated gene, vesicles

Functional category

• Signaling (Hedgehog, GPCRs, ion channels)

Function

DKK3, a secreted canonical Wnt regulator and WNT4, a noncanonical Wnt ligand act together to facilitate a canonical to noncanonical Wnt signaling switch during multiciliated cell formation. In primary human airway epithelial cells, DKK3 and WNT4 CRISPR knockout blocks, whereas ectopic expression promotes, multiciliated cell formation by inhibiting canonical Wnt signaling. Wnt4 and Dkk3 single-knockout mice also display defective ciliated cells. DKK3 and WNT4 are co-secreted from basal stem cells and act directly on multiciliated cells via KREMEN1 and FZD6, respectively(PMID: 37505110).