YAP1

Cilia effects upon perturbation of YAP1

Cilia number / % ciliated:

Decreased cilia number

Loss-of-function effect:

Shorter cilia

Overexpression effect:

Increased

Ciliogenesis screen results (4 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-8.17) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: Increased Signaling (Negative Regulator) PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

preweaning lethality, complete penetrance

Human ciliopathy phenotype:

uveal coloboma-cleft lip and palate-intellectual disability; Uveal coloboma - cleft lip and palate - intellectual disability; coloboma

Subcellular localization

cilia associated gene, cytosol, nucleus

Functional category

• Ciliary assembly/disassembly
• Actin & cytoskeleton regulation
• T cell biology
• Neurogenesis & migration
• Cardiac & muscle development
• Transcription regulation

Function

Transcriptio l co-activator with TAZ. Knockdown of YAP does not inhibiti ciliogenesis( 25367221) but is sufficient to induce ciliogenesis, whereas YAP/TAZ hyperactivation suppresses serum starvation- mediated ciliogenesis ( 25849865). Cytoplasmic retention of YAP/TAZ correlates with active ciliogenesis either in spatially confined cells or in cells treated with an actin filament destabilizer ( 25849865). Mutations in NEK8/NPHP9 affect YAP nuclear localisation, with different mutations having different effects, leading to major organ developmental defects due to altered ciliogenesis and cell differentiation/proliferation ( 26967905)