ZBTB17

Cilia effects upon perturbation of ZBTB17

Cilia number / % ciliated:

No effect

Loss-of-function effect:

No effect

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.50) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Pusapati et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, neg_rank=803, lfc=-2.29) PMID:30270045

Subcellular localization

cilia, nucleus

Functional category

• Ciliary assembly/disassembly
• Transcription regulation

Function

Activation of Smo induces translocation of Miz1 to the primary cilia together with Smo and Gli2. Furthermore, Miz1 is localized to the nucleus upon Hh activation in a Smo-dependent manner, and loss of Miz1 prevents the nuclear translocation of Gli2. Knockdown of Miz1 had no effect on ciliogenesis as primary cilia were detected in similar numbers of control and knockdown cells (Scr, 66%±3%, #3, 68%±3%, and #5, 60%±3%), and no changes in cilium length and width were observed. In the absence of stimulation, a slight but significant decrease of GFP-Smo accumulation in primary cilia was observed in Miz1 knockdown cells (23671675).