ZIC3

Cilia effects upon perturbation of ZIC3

Loss-of-function effect:

Shorter cilia

Ciliogenesis screen results (5 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-3.99) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381
• Elliott et al. 2025 (CRISPRa) [CRISPRa]: Disassembly Trigger (casTLE Effect=-5.49) PMID:41160700

Phenotypes

Human ciliopathy phenotype:

heterotaxy, visceral, 1, X-linked; VACTERL association, X-linked, with or without hydrocephalus; congenital heart defects, multiple types, 1, X-linked; visceral heterotaxy; congenital heart malformation

Ciliopathy associations

• Visceral Heterotaxy / Situs Inversus

Subcellular localization

cilia associated gene, nucleus

Functional category

• Ciliary assembly/disassembly
• Cardiac & muscle development
• Transcription regulation

Function

Mutations in the ZIC3 gene cause Visceral Heterotaxy (PMID: 9354794).