ZNF676

Cilia effects upon perturbation of ZNF676

Cilia number / % ciliated:

Unknown

Loss-of-function effect:

Unknown

Overexpression effect:

Shorter cilia

Ciliogenesis screen results (1 screen)

• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680

Subcellular localization

cilia associated gene

Functional category

• Ciliary assembly/disassembly

Function

Both immunofluorescence enhanced microscopy and scanning electron microscopy revealed profound perturbations of ciliogenesis in ZNF676-overexpressing cells, characterized by abnormally short and disorganized cilia . A comparison of the transcriptomes of these cells and their LacZ-transduced controls revealed that a large fraction of differentially expressed genes were related to the cilium. Primary normal human broncho-epithelial (NHBE) cells with lentivectors harboring dox-controllable versions of ZNF676 or LacZ and induced the expression of these transgenes upon triggering differentiation into ciliated cells at an air-liquid interface. (PMID: 35042549).